B I O M E D I C A L   A P P L I C A T I O N


 MEMS based Cell manipulator & exciter

Micro Systems Lab. in AJOU UNIVERSITY

Fig. 1. The structure of the micro cell exciter actuated by the electromagnetic force.

Fig. 2. Analysis of histology (H&E)

 Cell exciter actuated for the chondrogenic differentiation of mesenchymal stem cells
W. Y. Sim, S. W. Park, S. S. Yang, S. H. Park, and B. H. Min
The stem cells have remarkable potential to develop into many different cell types in the body.  Therefore, it can be applied to the medical therapies associated with bone marrows, nerve cells, heart muscle cells and pancreatic islet cells.
Among the stem cells, bone marrow-derived mesenchymal stem cells (MSCs) attract worldwide attention as a source of easily isolation, regenerative potential, and geneticplasticity.   Previous studies have shown that MSCs reproducibly and predictably differentiate into bone, cartilage, adipose, and other tissues in vitro and in vivo.  The differentiation into cartilage from MSCs can be applied to develop a replacement therapy for damaged or diseased cartilage. MSCs require appropriate signals to differentiate specifically into cartilage.  The biochemical factors have been investigated to find the mechanisms of differentiation of stem cells.  Recently, several groups reported that physical and mechanical stimulations play another important role to differentiate MSCs.  However, these methods of traditional experiment have several drawbacks, such as the large quantity of sample, the low reliability, and the low reproducibility. Compared with the previous researches, the micro cell exciter of this paper has the two key merits such as the reduction of the necessary quantity of MSCs and the increment of the reliability about a partitioning and quantification of MSCs.  Several kinds of stimulation are tested to identify the effective stimulation in the chondrogenic differentiation of MSCs.  Histological assay and reverse transcriptase (RT)-PCR analysis were performed to observe the expression of the chondrogenesis.

Fig. 3. The Analysis by RT-PCR.  (a) bare condition (b) Static MF condition (c) Dynamic MF condition
(d) Static MF and static PR condition (e) Dynamic MF and dynamic PR condition.


  Related Papers

  1. 박신욱, 심우영, 박상혁, 박소라, 민병현, 양상식, “토끼 중간엽 줄기 세포의 연골세포 분화를 위한 전자력구동 세포 자극기의 제작 및 시험,” 제6회 한국 MEMS 학술대회 논문집, pp. 78-82, 2004. 04.
  2. Sin Wook Park, Woo Young Sim, Sang Hyuk Park, Byung Hyun Min, and Sang Sik Yang, "Fabrication and test of a micro cell exciter for the differentiation of mesenchymal stem cells (MSCs)," International Conference on Electrical Engineering 2004 (Joint Conference with APCOT MNT 2004), Sapporo, Japan, pp. 272-275, 2004. 07.
  3. 박상혁, 심우영, 박신욱, 박소라, 양상식, 민병현, “전자력 구동 세포 자극기를 이용한 토끼 중간엽 줄기 세포의 연골화 분화,” 제30차 대한정형외과연구학회 학술대회, p. 47, 2004. 10.
  4. Sang Hyug Park, Woo Young Sim, Sin Wook Park, Sang Sik Yang, So Ra Park and Byung Hyun Min, "Chondrogenic differentiation of rabbit mesenchymal stem cells (MSCs) by an electromagnetic force cell exciter," Joint Meeting of the Tissue Engineering Society International and the European Tissue Engineering Society, Lausanne, Switzerland, p. 236, 2004. 10.
  5. W. Y. Sim, S. W. Park, S. S. Yang, S. H. Park, and B. H. Min, "Fabrication and Preliminary Test Results of a MEMS Cell Stimulator for Stem Cell Research," 1st IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2006), Zhuhai, China, pp. 154,2006. 01.
  6. 심우영, 박신욱, 양상식, 박상혁, 민병현, 박소라, “공압 방식 세포자극칩을 이용한 인간간엽 줄기세포의 자극 시험,” 제8회 한국 MEMS 학술대회 논문집, pp. 1-4, 2006. 04.
  7. W. Y. Sim, S. W. Park, S. H. Park, B. H. Min, S. R. Park, S. S. Yang ,  "A Pneumatic Micro Cell Chip for the Differentiation of Human Mesenchymal Stem Cells (hMSCs) under Mechanical Stimulation" , Lab on a chip, volume 7, issue 12, pp. 1775-1782, 2007. 12

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