B I O M E D I C A L A
P P L I C A T I O N
MEMS based Cell manipulator & exciter
Lab. in AJOU UNIVERSITY
1. The structure of the micro
cell exciter actuated by the electromagnetic force.
2. Analysis of histology (H&E)
Cell exciter actuated for the chondrogenic
differentiation of mesenchymal stem cells
W. Y. Sim, S. W. Park, S. S. Yang, S. H. Park, and B. H. Min
The stem cells have remarkable potential to develop into many different cell types in the body. Therefore, it can be applied to the medical therapies associated with bone marrows, nerve cells, heart muscle cells and pancreatic islet cells. Among the stem cells, bone marrow-derived mesenchymal stem cells (MSCs) attract worldwide attention as a source of easily isolation, regenerative potential, and geneticplasticity. Previous studies have shown that MSCs reproducibly and predictably differentiate into bone, cartilage, adipose, and other tissues in vitro and in vivo. The differentiation into cartilage from MSCs can be applied to develop a replacement therapy for damaged or diseased cartilage. MSCs require appropriate signals to differentiate specifically into cartilage. The biochemical factors have been investigated to find the mechanisms of differentiation of stem cells. Recently, several groups reported that physical and mechanical stimulations play another important role to differentiate MSCs. However, these methods of traditional experiment have several drawbacks, such as the large quantity of sample, the low reliability, and the low reproducibility. Compared with the previous researches, the micro cell exciter of this paper has the two key merits such as the reduction of the necessary quantity of MSCs and the increment of the reliability about a partitioning and quantification of MSCs. Several kinds of stimulation are tested to identify the effective stimulation in the chondrogenic differentiation of MSCs. Histological assay and reverse transcriptase (RT)-PCR analysis were performed to observe the expression of the chondrogenesis.
Fig. 3. The Analysis by RT-PCR. (a) bare condition (b) Static MF condition (c) Dynamic MF condition
(d) Static MF and static PR condition (e) Dynamic MF and dynamic PR condition.
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- Sang Hyug Park, Woo Young Sim, Sin Wook Park, Sang Sik Yang, So Ra Park and Byung Hyun Min, "Chondrogenic differentiation of rabbit mesenchymal stem cells (MSCs) by an electromagnetic force cell exciter," Joint Meeting of the Tissue Engineering Society International and the European Tissue Engineering Society, Lausanne, Switzerland, p. 236, 2004. 10.
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- W. Y. Sim, S. W. Park, S. H. Park, B. H. Min, S. R. Park, S. S. Yang , "A Pneumatic Micro Cell Chip for the Differentiation of Human Mesenchymal Stem Cells (hMSCs) under Mechanical Stimulation" , Lab on a chip, volume 7, issue 12, pp. 1775-1782, 2007. 12
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